Hybridomas producing the monoclonal antibodies to the cotton membrane proteins
نویسندگان
چکیده
منابع مشابه
Human-human hybridomas producing monoclonal antibodies of predefined antigenic specificity.
We report the establishment of human-human hybridomas producing monoclonal antibody of predefined antigenic specificity. The U-266 human myeloma cell line was incubated in the presence of 8-azaguanine, and a rapidly growing, 8-azaguanine-resistant, hypoxanthine/amethopterin/thymidine (HAT) medium-sensitive mutant line, U-266AR1, was selected. These cells were fused with lymphoid cells from unin...
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Two toxic substances are produced, a hemolysin (Tetanolysine) and the potent lethal toxin (Tetanospasmin or Neurotoxin). The former is responsible for areas of hemolysis around colonies on blood agar plates. Neurotoxin is highly toxic when injected parentrally; however, it is harmless if administered by mouth. Animals vary in their susceptibility to tetanus toxin, e.g., horse and man are the mo...
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Antibodies containing two different antigenbinding sites were prepared for the first time in 1961 by Nisonoff and Rivers [1] by dissociating two different antibodies and reassociating the mixture of half molecules. But this method of chemical recombination has some disadvantages because it results in protein denaturation and therefore loss of antibody activity. The production ofbispecific antib...
متن کاملDetection and characterization of monoclonal antibodies to platelet membrane proteins
We have devised a solid-phase radioimmunoassay for the detection and characterization of monoclonal antibodies directed against platelet surface antigens. Platelet membrane proteins, solubilized with 0.1% Triton X-100, were covalently coupled to cyanogen bromide (CNBr)-activated filter paper disks that were than used as the support in antibody binding assays. SDS PAGE of solubilized membrane pr...
متن کاملMonoclonal Antibodies
SUMMARY Monoclonal Antibodies are identical antibodies and Can be generated in ultimated amounts by countinuos cultures of single antibody- secreting cells. These cell lines are produced by cell fusion of normal lymphocytes to cells of a myeloma tumor cell line, which confers on the antibody producing hybrid cell, immortality and the ability grow as atumor in animals. monoclonal antibodies c...
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ژورنال
عنوان ژورنال: Biopolymers and Cell
سال: 1999
ISSN: 0233-7657,1993-6842
DOI: 10.7124/bc.000526